How it works
These high school biology questions are unofficial practice: 129 biology multiple choice questions of our own, sorted into nine topics. None is taken from a school, state or national exam.
Read the full guide
Nine topics
Molecules of life, cells and membranes, photosynthesis and respiration, cell division, genetics, DNA and proteins, evolution, ecology, and the body in balance. Choose one before a unit biology test, or leave them mixed for a biology exam review.
Where the facts come from
The topic list follows the high school life science standards of the Next Generation Science Standards (HS-LS1 to HS-LS4), read October 7, 2026. Facts were checked on NIH glossaries, MedlinePlus Genetics, NOAA, EPA and U.S. Geological Survey pages, named under each question.
Exam mode
Exam mode is a biology practice exam of 25 questions drawn from the whole bank, scored at the end. The 25 is our number, not the length of any real exam.
- Miss a question twice? Open the source link under it and read the whole entry, not only the line you needed.
- For a genetics cross, write the four outcomes down before you read the choices.
Questions and answers
Are these biology questions and answers free?
Yes, with no sign-up. Every question shows the right answer and a short reason as soon as you choose, with a link to the page the fact was checked on.
Is there a biology practice test PDF?
Yes. After a round, press Print, then Add all to put the whole bank on the sheet, with the answer key if you tick it. In the print window, choose Save as PDF to keep it.
Will this help with a biology placement test?
It can help you review. Each college sets its own biology placement test and decides what is on it, so ask the testing office what yours covers. This bank is the high school course.
Is this an honors biology practice test?
Each school sets its own honors course, so no bank matches it exactly. The harder questions here, on mutations, inheritance odds, gene regulation and feedback, are the closest to an honors class.
What topics are on a high school biology exam?
Each school or state sets its own. This page follows the Next Generation Science Standards for high school life science: structures and processes of organisms, ecosystems, heredity, and evolution. For a state end-of-course test, read that state's own blueprint.